Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHN----YITLLDNIQRERWVMLAGAVLGIATSILLFNTMNRLYFEE-----------FRRAIFIKRIAGL----RFLEIHRTYLFAQLGVFLLGFVASVFLQVEIGVAFLVLLLFT--GLSLLQLHVQMQKENKMSMLVLKGG-- 4PWO Chain:A ((1-250)) ----------------------TQAEKTATDLPPGFSGKAGSVNATPGTKGLDGPTPLADGSFDATIFGPAKELKSADDILNVHRRNAKDP----FAVGAVDAPLVITEFSDFECPFCARWSNQTEPTLMEEYVSKGLVRIEWNDLPVNGEHALAAAKAGRAAAAQGKFDEFRKALFEASRNVSGHPNNTLKDFERFARNAGVKDMERFSREAQDSTYDEVLAKAADYAHG--LGVSGTPAFVVGTQYISGAQPTEEFIKVIESELKKSPTFSTPSSH

General information: TITO was launched using: RESULT: Template: 4PWO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1108 -19340 -17.45 -85.20 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -17.45 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_4PWO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PWO-query.scw PDB file : Tito_Scwrl_4PWO.pdb: