Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCEFISEYSEFQYD----FISLAQQRFVSVPEIDLRTMIQNFGDWYFANESSSLNTPSIWLVKWFSWVQNNEKQVAANRKKQEQITSTCQKPQESGYFAN----LFEEQSESQIVDVTPAKKFPMIEEVGHA 2Z4T Chain:A ((317-425)) ---------QMQWDGFKKFNSKQQELFLS---------IVNFDKQKLQNEYNSSNLPNFVFTGTTVWAGNHEREYYA--KQQINVINNAINESSPHYLGNSYDLFFKGHPGGGIINTLIMQNYPSMVDI---

General information: TITO was launched using: RESULT: Template: 2Z4T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 241 -1653 -6.86 -16.36 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -6.86 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.095

(partial model without unconserved sides chains): PDB file : Tito_2Z4T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z4T-query.scw PDB file : Tito_Scwrl_2Z4T.pdb: