Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISLSSFQIGS----------FFALCSAFLFSTKAIF-------------IKQTYALSPLVDGTVLMAL-----------RMLSALPFFLLICWFNRHHNQGIKKKD--WLILIFAGLLGYYFASWLDFMSLMFISAS---------------LERIILFLYPTLTVIASSLL---------------YKQKLDVKSLFAIFLSYGGTVLVMLQEHNNATIQGKFWLGTSFVFASALA----------FAGYLLLTPPLIKKFGSWNFTG--------LALTVACIGTLTHYTLSTSHPFQLLLQLPSSVIWYGIGLGFLVTVLPTVMLMQSIERLGASQSAMIASIGPVLTILLAVAFLNEHLNIWQWVGCFLNIIGVMMITLRKKKLKH------- 5EC0 Chain:A ((1-388)) MNISRMNVDFGNSMYMNLIDGYFFELPTNVVEISKEAAEGKFTSIVEDPADLKDRLLVSTVIDETERYFLVGELAEPELHNKVESHIPYVTFLAATAYYQALKGKREDNEVTIEYFQTMLPIWLLKKLDKFSEMQKRMASKFLGTHQVKVLTLGLEKELTIKVEDAACRIESEVARWAIKKNFDLEDKDYAEQFKNYDVVFCDLGGGTDDLVLLPAGLKPPKSRDSFVSNTAPFLAHLEKLRKEKLLEHFDSVRELEKFIYSNIGKTKMERRDGNTGQKFDLTDIIKKSLKEYTEIKIAQAENTFPAPKDKVYKYLYFGGVGEVLEESISVVTEERYGRDISESN-------HIVAEDARL-------------LNLYGLEVLSRAEQVKKQANEKEAQ

General information: TITO was launched using: RESULT: Template: 5EC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1318 -68629 -52.07 -238.30 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -52.07 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.246

(partial model without unconserved sides chains): PDB file : Tito_5EC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EC0-query.scw PDB file : Tito_Scwrl_5EC0.pdb: