Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDGAMQGSYATALLKQQQLPESFQFYFKQKQHLSNTQELHQLHELVRPRLKIAIVTETWPPEINGVALSLLQLCQGLQKQGHKILLVRPEQKAKCHDFLPEQECLVMSQAIP-----KYPTLQFGWPQYLKVSKAFEKFVPDVVHI--VTEGPLGLTAMQAAKAKDIPVSSGFHSPFQDFSRFFDLAFLVKPIQKYLCWFHNNTQVTCVPSKDTEEALRGFGITCPLVVVGRGVDTTRFSPKHRSENLRQQWGVDSDTRVMLYVGRLS-PEKEVQLIVESYAAMQNIQQQ--KTKLVVVGDGP-DLARLKALPEAKNVIFTGSLCGHDLAVAYASADVFVFAS-QVETFGNVVLEAMASGLPVIAYDYACAHQYLIHGVNGWLSPLGHKNHFIQQIYQLPSIQQLREMGI-QACHKVQQSGWQLPVQQLEQAFYQVVKELSADFT 4N9W Chain:A ((19-367)) ---------------------------------------------------------------GGVQSHVLQLAEVLRDAGHEVSVLAP---ASPHVKLPDY-VVSGGKAVPIPYNGSVARLRFGPATHRKVKKWIAEGDFDVLHIHEPNAPSLSMLALQAAEG---PIVATFHT---STTKSLTLSVFQGILRPYHEKIIGRIAVS--------------AVEIP-----NGVDVASFADAPLLD------GYPREGRTVLFLGRYDEPRKGMAVLL---AALPKLVARFPDVEILIVGRGDEDELREQAGDLAGHLRFLGQVDDATKASAMRSADVYCAPHLGGESFGIVLVEAMAAGTAVVASDLDAFRRVLADGDAGRLVPVDDADGMAAALIGILEDDQLRAGYVARASERVHRYDWSVVSAQIMRVYETV---------

General information: TITO was launched using: RESULT: Template: 4N9W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1595 -36386 -22.81 -113.00 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -22.81 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_4N9W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N9W-query.scw PDB file : Tito_Scwrl_4N9W.pdb: