Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKSVTQENSVPVIGQRFRGFLPVVVDVETAGFNAQTDALLEIACIPIVYDAQGQFVPGPAFHAHINPFEGANLDRRSLDFIGIDPFNPMRIAMAEDERTALRRIFKSVNEVRKQQHCTHAVLVGHNAHFDLGFLQAAIARSGTKNQNPFHSFSVMDTVTLSAVMFGQTVLAKACIQAGIEFDGKEAHSALYDTQKTAELFCYILNKLSPYLLDSLVAAS 3VA0 Chain:A ((33-221)) ----------------RFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYE--ALHEIFKVVRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLK-RNPFHPFATFDTAALAGLALGQTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRW------------

General information: TITO was launched using: RESULT: Template: 3VA0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 988 -132301 -133.91 -700.01 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -133.91 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_3VA0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VA0-query.scw PDB file : Tito_Scwrl_3VA0.pdb: