Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTAVANVKLNWTYFCLILASTFLQGSSFVATKILLSSMSPLWVATIRFFIAALSLLPLIIYRYFKNPISLFNIPWLKLFVIGLFQTAGVMAFLNIGLGYTSPSTAAILMASNPLLVVILAMLILGERISIRALVGLIVAFIGVVICIGLGNTN---SGGIGRGEVLVILASSCWAIATIINKKFNLHLDPWVITFWQMLLGSLVLFLVALFSQQPFTLPTTESMWLTFLWLAIPASTGAMGLWFAALKIGGAIHTSGFLFLCPLFSAIITYFVLGTVLTSQELIGGFLIGTGIYVLSRYR 5I20 Chain:D ((2-284)) ----------SRSSATLIGFTAILLWSTLALATS-STGAVPPFLLTALTFTIGGAVGIAAGLARGV-G----LRQPWP-VWVHGIGGLFGYHFFYFSALKLAPPAEAGLVAYLWPLLIVLFSAFLPGERLRPAHVAGALMGLAGTVVLLGA-AGGFGFAPEYVPGYLAAAACAVIWSVYSVASRRFAR-VPTEVVAGFCLATAALSALCHILFEP-S--VWPVGSEWLAVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPVLSTLLLVVAGFAAPSGALAIACALIVGGAAVATLL-

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1358 -245003 -180.41 -887.69 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -180.41 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.342

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: