Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYSEKVIDHYENPRNVGVLDKNSENVGTGMVGAPACGDVMRLQIQVNDNGVIEEARFKTYGCGSAIASSSLVTEWLKGKTLDEAQAIKNIDIATEL-ALPPVKVHCSVLAEDAIKAAIEDYRSKKSKA 4EB5 Chain:C ((2-123)) --YSDKVFDHFQNPRNVGKIE-DADGVGT--VGNPVCGDLMTIYIKVKDN-RIEDIKFQTFGCAAAIATSSMATEMAKGKTIEEALKITRDAVAEALGGLPKQKMHCSNLAADALRRAIVDYFRKNGK-

General information: TITO was launched using: RESULT: Template: 4EB5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 652 -42821 -65.68 -353.89 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : -65.68 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.575

(partial model without unconserved sides chains): PDB file : Tito_4EB5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EB5-query.scw PDB file : Tito_Scwrl_4EB5.pdb: