Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNVSMTQRISEVVRNTNETKIRVRLNLDGTGQGTLNTGVPFLDHMIDQIKQHGLFDIDIHCDGDLEIDDHHTVEDCGITLGQAFAQALGDKKGLRRYGHFYAPLDEALSRVVVDLSGRPGLFMDIPFTRARIGTFDVDLFSEFFQGFVNHALMTLHIDNLKGKNSHHQIESVFKALARALRMACEIDPRAENTIASTKGSL 4MU1 Chain:A ((21-205)) ------SARIGEVKRETKETNVSVKINLDGHGVSDSSTGIPFLDHMLDQLA-HGLFDVHVRATGDTHIDDHHTNEDVALAIGTALLKALGERKGINRFGDFTAPLDEALIHVSLDLSGRPYLGYNLEIPTQRVGTYDTQLVEHFFQSLVNTSGMTLHIRQLAGKNSHHIIEATFKAFARALRQATESDPRR-----------

General information: TITO was launched using: RESULT: Template: 4MU1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1027 -117461 -114.37 -638.38 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -114.37 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.428

(partial model without unconserved sides chains): PDB file : Tito_4MU1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MU1-query.scw PDB file : Tito_Scwrl_4MU1.pdb: