Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYDDQNIFAKILRGELPAIKLYEDDQVLAFMDIMPQADGHALVIPKTPAVTLLDLPPEAAAYTIQIVQKIAKAMETALNLDGIVLMQLSGAAAGQTVPHVHFHLIPTNVHQLGKHAAQLGDQDKIKALAEKIKAAL 4ZGL Chain:A ((2-101)) ------NVFEKIIQGEIPCSKILENERFLSFYDINPKAKVHALVIPKQSIQDFNGITPELMAQMTSFIFEVVEKL--GIKEKGYKLLTNVGKNAGQEVMHLHFHILSG-----------------------------

General information: TITO was launched using: RESULT: Template: 4ZGL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 417 -78460 -188.15 -784.60 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -188.15 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.697

(partial model without unconserved sides chains): PDB file : Tito_4ZGL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZGL-query.scw PDB file : Tito_Scwrl_4ZGL.pdb: