Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEFSIIDQYFNRQSHPDVALGIGDDSALITPPPNQQLVICADTLVAGRHFPLETLSHAIGWKSVAVNLSDIAAMGAKPHSILLAISLPQ-VDHEWLEGFSQGIYDCCNQFGVALIGGDTTQGPHLTITVTAMGWIETGKAVLRSGAKVGDYVCVSGQIGDAAYGLQHLGH----------SLQQRLDYPTPRCKLGEELKGLASSMIDVSDGLAQDLGHILKASKVGARLILEKLPVDPVLQQLEE---QQRWQYALAGGDDYELCFTITPQNYEKLLQKQLDVKITMIGQIVEQTKLTFEHLGSDYPLQIHGYQHFA 3C9T Chain:A ((42-323)) LGEFGLID-LIKKTLESKV---IGDDTAPVEYC-SKKLLLTTDVLNEGVHFLRSYIPEAVGWKAISVNVSDVIANGGLPKWALISLNLPEDLEVSYVERFYIGVKRACEFYKCEVVGGNISKSEKIGISVFLVG--ETERFVGRDGARLGDSVFVSGTLGDSRAGLELLLMEKEEYEPFELALIQRHLRPTARIDYVKHIQKYANASMDISDGLVADANHLAQRSGVKIEILSEKLPLSNELKMYCEKYGKNPIEYALFGGEDYQLLFTHPKERWNPFLD------MTEIGRVEE------------------------

General information: TITO was launched using: RESULT: Template: 3C9T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1411 -17145 -12.15 -63.97 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -12.15 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_3C9T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C9T-query.scw PDB file : Tito_Scwrl_3C9T.pdb: