Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIHLFFRNNKKSKMHLKLNNYLISGEYMNIPRFGLGTFRLKEQAVIDSVKNALDVGYRAIDTAQIYENEAAIGQAIAESGVSRQDLFLTTKIWVDNFAQDKFIPSLKESLQKLRTDHVDLTLIHWPAPDLGVSIPEIMQLLLEAKQQGLTKQIGISNFNIALTQQAIDTIGVEHIATNQIELSPYLQNHKLVNFLQEKNIDVTSYMTLAYGKVLQDPVLAEIAAANKATTAQIALAWALQRGFAVIPSSTKRENLISNLKAQDIELTAAEMQMIAELDRNSREVSPEPWAPVWD 3WBY Chain:A ((25-277)) ------------------------------MPQIGLGVWETPPDETAEVVKEAVKLGYRSVATARLYKNEEGVGKGLED----HPEIFLTTKLWNDEQGYDSTLRAYEESARLLRRPVLDLYLIHWPMPAQGQYV-ETWKALVELKKSGRVKSIGVSNFESEHLERIMDATGVVPV-VNQIELHPDFQQRALREFHEKHNIRTESWRPLGKGRVLSDERIGKIAEKHSRTPAQVVIRWHLQNGLIVIPKSVNPKRLAENLDVFGFVLDADDMQAIEQMDRKDGRMGADP------

General information: TITO was launched using: RESULT: Template: 3WBY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1330 -107222 -80.62 -423.80 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -80.62 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_3WBY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WBY-query.scw PDB file : Tito_Scwrl_3WBY.pdb: