Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITQNKDILFRSPIMNKLMVYAYTGVVITMLFWGSAFNAMSYVIQYMPPLSAAAERFTIASLGLFILFAAIGKLSWAALRQNLVIYLIIGIIGIAGFNLGCFYGLQTTSAVNGALIMATTPLITLLLTILLDGEKLTLNKFIGVVFGLSGVLFVISHGHISTLLHLKIAIGDLFILLGGVSFCLANVLSRRYVKNATPLETTTFSMMFGALTLIILSVIFEHPSQAIAMAPITAHLAMGYVIICSTMIAYLFWFNGIQKLGAGRASIFFNFVPVFSMLVALLAGQSLNIWQLIGTALVMLGVMSSGGFIQIKRPALIAKPCTK 5I20 Chain:D ((147-285)) ----------------PEYVPGYLAAAACAVIWSVYSVASRRFAR-VPTEVVAGFCLATAALSALCHILFEPS--VWPVGSEWLAVVALGIGPVGIAFYTWDIGMKRGDVRLLGVLSYAAPVLSTLLLVVAGFAAPSGALAIACALIVGGAAVATLLA---------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5I20.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 233 -35215 -151.14 -253.35 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.51 3D Compatibility (PKB) : -151.14 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.51 QMean score : -0.079

(partial model without unconserved sides chains): PDB file : Tito_5I20.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I20-query.scw PDB file : Tito_Scwrl_5I20.pdb: