Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQPNDYSKIYDIAVIGGGINGVGIANDAVGRGLSVFLCEKDDLASHTSSASSKLIHGGLRYLEYKEFRLVREALAEREVLLAKAPHIIKPMRFIMP----HRPHLRPAWLIRAGLFFYDHLGK------REKLLGSNRIYFKEDSPLKPAITRGFEYSDCTVDDARLVVLNALQAKEKGAKVVTRKRCVK-AYRQQELWYLELQS--GAEFYQVRAKAIVNAAGPWVEEIISENLNLSSPY--QIRLIQGSHIVV---------PKLYDCHKAFIMQNEDRRIVFAIPYLEKYTLIGTTDQEYTGDPQKVEITDVEIDYLLTVTNSHFKK-QLTRADIVSQYSGVRAL------------CDDESDNPSAITRDYTLALQAEDKTTPLLSVFGGKITTYRKLAEAALEHLAPFFNDMAEEWTADDPLPGAENW---------TTLEDLINQIKTR--VSGISDSLANRWAHAYGT---RVWNMLKERNAIEQLGQHFGHDLFE-CEVRYLCEYEWAHTAEDILWRRSKLGLAFDEKQVKVLEAYLSERRLKDDAA 2RGO Chain:A ((41-552)) ---------------------------------GIKTGLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQGIAPHIPKPDPMLLPIYEDEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGLKGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYEGDQIVGVKARDLLTDEVIEIKAKLVINTSGPWVDKV--RNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFDTGK------QDGRMVFAIPR-ENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANITLADIEASWAGLRPLLIGNSGSDYNGG---------TISRGSSL----EREPDGLLTLSGGKITDYRKMAEGALRLIRQL---LKEEYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNARRIFELAKE-------APYPGLSLAESARLRYGLEEEMVLAPGDYLIRRT----------------------------

General information: TITO was launched using: RESULT: Template: 2RGO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1989 40170 20.20 97.50 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 20.20 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.342

(partial model without unconserved sides chains): PDB file : Tito_2RGO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RGO-query.scw PDB file : Tito_Scwrl_2RGO.pdb: