Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFASILRELNYLRSNKWDSCMVTLIPAFIIFLFSSMFYAGKAEHLPIAIIDQDQSELSRNIGKYLSLNHTVDVKTITTSSSEAERL----LNETKIWGYIFIPDGAEKRFVKAQDAQISIAFNQSFFSVGNTI-SSAM--------LVSTLNALADY-AGQNYLANN-I--PYLDVPTAHVKI------STLYNPSMSYEFYLEPFMVPAVLHLLLCCCVAFAIGQEIKRGTLTQW-VNRESFIQGLLAKNLLYSFIFCFWTWLWMFWLVEIRGWFVAGSLSFLLAAQFLLYFSYALISSTVILATKNLSKTFGFIAVYGGSSLSFAG-VTLPLNNAPIFTKFWSL-IIPYTPYAKLQT-------EQWVIGSPLFISMSPFLILIGYCLIYFFLSCLLLKKLVQGATP 5XJY Chain:A ((533-1119)) ---------------------------------------------------------------------------PIATEVWLINKSMELLDERKFWAGIVFTGITPGSIELPHHVKYKIRMDIDNVERTNKIKDGYWDPGPRADPFEDMRYVWGGFAYLQDVVEQAIIRVLTGTEKKTGVYMQQMPYPCYVDDIFLRVMSRSMPLFMTLAWIYSVAV-IIKGIVYEKEARLKETMRIMGLDNSILWFSWFISS-LIPLLVSAGLLVVILKLGNLLPYSDPSVVFVFLSVFAVVTILQCFLISTLFSRANLAAACGGIIYFTLYLPYVLCVAWQDYVGFTLKIFASLLSPVAFGFGCEYFALFEEQ------------GFNLTTSVSMMLFDTFLYGVMTWYIEAVF--

General information: TITO was launched using: RESULT: Template: 5XJY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 823 -89271 -108.47 -313.23 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -108.47 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.183

(partial model without unconserved sides chains): PDB file : Tito_5XJY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5XJY-query.scw PDB file : Tito_Scwrl_5XJY.pdb: