Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFRNNVPQASWRLAKIALGGIVLTGLAVGTYAYAQQKPLPTVDKVELDRYLGVWYEVARKPAFFQK-KCAYNVSATYTLNENGNIVVDNRCYDNQKQLQQSISEAFVVNPPYNTKLKVSFLPEAVRWIPIIRGDYWILKLD-EDYQTVLVGEPSRK-----YLWVLSRTPHPHKEVVDEYLNYAKTLGFDIRDIIHTEYKE 2HZR Chain:A ((12-161)) ----------------------------------------PVQENFDVNKYPGRWYEIEKIPT----GRC---IQANYSLME-GKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKF-----SWF-MPSAPYHILATDYENYALVYSCTSISQSFHVDFAWILARNVALPPETVDSLKNILTSNNIDVKKMTVTD---

General information: TITO was launched using: RESULT: Template: 2HZR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 592 7300 12.33 52.90 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 12.33 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_2HZR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HZR-query.scw PDB file : Tito_Scwrl_2HZR.pdb: