Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALTEKKKAFALAKRNGKDNKEAAILAGCPEKTASAAGARLAKDPDVIAYLERLEEATPEQAVKHEVKPLTTFTSIQTANKLDDPLEFLKSVY----TDQVEDMALRVR--------AAQAALPYVHGKVAEKGKKETKEDAAKAATKTGKFGTLNNQLPS 5TD8 Chain:C ((4-62)) -----------------------------------------------------------------------------KDNLLDNPVEFLKEVRESFDIQQDVDAMKRIRHDLDVIKEESEARISKEHSKVSESNKK-------------------------

General information: TITO was launched using: RESULT: Template: 5TD8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 15 950 63.30 20.20 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : 63.30 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.306

(partial model without unconserved sides chains): PDB file : Tito_5TD8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TD8-query.scw PDB file : Tito_Scwrl_5TD8.pdb: