Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHMVTTKKAKVQFNWQDPFLIEQQLSPDERMIHDAAHAYCQERLQPRVLEQFRHETTDPSIFREMGELGLLGPTIPEQYGGAGLNYVSYGLVAREIEYVDSGYRSMASVQSSLVMVPIHEFGTEEQKQKYLPKLATGEYIGCFGLTEPDHGSDPGSMITRA--KKVEGGYRLTGAKMWITNSPIADVFVVWAKEVSAEGNVGDICGFILEKGWEGLSAPAIHGKVGLRASITGEIVMDNVFVPEENAFPEIRGLKGPFTCLNSARYGIAWGAMGAAEFCWHTAHQYTMDRKQFGRPLAANQLIQKKLADMQTEIALGLQAALRFGRMKDEGIASVEGTSLIKRNNCGKALDIARMARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDVHALILGRAQTGIAAFSN 2R0N Chain:A ((3-391)) ------------EFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTI-KGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWAR-----CEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFT-

General information: TITO was launched using: RESULT: Template: 2R0N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2197 -76274 -34.72 -197.09 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -34.72 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_2R0N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R0N-query.scw PDB file : Tito_Scwrl_2R0N.pdb: