Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRTPISFEFFPPKTDASAEKLRIVHQELQHLNPEFFSITYGAGGSTRERTLAAIEDFNGK-GTPVAPHLSCIGDDKTRIAELLDLYKAQGIDRIVALRGDLPSGQVGLGELPYAQDLVRFIREHSGDHFHIEVAAYPEMHPQAESLDSDIQRFIEKVQAGANAGITQFFFNPDSYFYFIERLEKAGINIPVAPGIMPITNASNLIRFADGTGAEIPRWIRKQLQAYGDDSESIKAFGHEVVVKLCERLIAGGAPSLHFYSMNQVEPTRQLVVDLGLN 1ZP4 Chain:A ((25-292)) ------VSFQFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYG-------RTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTIARDYWNNGIRHIVALRGDLPPG----PEM-YASDLVTLLKEVAD--FDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYTLNRAEMSYAICHTLGV-

General information: TITO was launched using: RESULT: Template: 1ZP4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1270 -44335 -34.91 -172.51 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -34.91 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.693

(partial model without unconserved sides chains): PDB file : Tito_1ZP4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZP4-query.scw PDB file : Tito_Scwrl_1ZP4.pdb: