Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNILDEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAAS-QSWKKIYQPLKIPRTIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYTDLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPNLTKWLKKESKICLFAADYRLLSVDEQ------DDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQLLAGEHIGSGLGILTS 4MFQ Chain:A ((134-334)) ---------------------------------------------------------------------------------VYVFLATVPAVRRFHAERGIPDEVGWETLTQLGELVAIHRRKYGQGGMNMQWWTTYHLRGILYRLGRLQFSLATGKDGTPHLGLAVPEWGGPLLPKAYDESLHRARP-------FFDRHFPEHGARVAWGSSWMLDPQLEEYLTEDSNIIQLARFWTLTDSAPEPGNADGDSSILEFVFRYNGQPLDELPQRSSLERAVIAHLKAGRH----------

General information: TITO was launched using: RESULT: Template: 4MFQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 686 -19530 -28.47 -100.67 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -28.47 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.369

(partial model without unconserved sides chains): PDB file : Tito_4MFQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MFQ-query.scw PDB file : Tito_Scwrl_4MFQ.pdb: