Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLTDILVCLSEKQNGRRNNMSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALVGNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESY-------TY--NDYFQIGMIGMIVSLVVVLTVANISMNKKKRY-AMA--A--N--V-PAEGGDAPMISWLAVLLPVLGVVLLD-------------LPI-I---LGFILAGIWALLFTGKLRGGYKEIC--RQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDWIP-----Q-SPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLF------PLFAITTIAP---QHLDITQSWVAWGLGYTK-----VTSREYMKKSIPTGWIIGAILCLITFLLYGNA 4R0C Chain:C ((72-484)) -------------------------------------------------------------------------------------TLVGNFTGFAPLG-VVLVA-MLGLGVAEQSGLLSVSLASLVRRSSGGALV---FTVAFAGVLS-SLTVDAGYVVLIPLAGLVFQLAGRPPIAGIATAFAAVSGGFSANLLVGP--VDATLAGLSTE-AAHIIDPDRTVAATGNYWFIIASTFLVTGLVTLITRTLTEPRLAHANTVADASVDAPQIHSRAMKWTGLTLAILLAGLAL-LVLPNDAPLRHPDTGSVLGSPFIHGLVVIVALIAGIC-GAVYGRVSGQFRNSGAVITAMEVTMASMAG-YLVLMFFAAQFVAWFNYSQLGLLLAVKGAAWLGALTVPKVVLLLLFVVLTAL------INLM-IGSASAKWSILAP-VFIPMLMLLGISPEASQAAYRVGDSSTNIITPLMPYFVLVLGFARRYQPETGIGTLIALMLPYSLTLLLGWSVLLGVWI---

General information: TITO was launched using: RESULT: Template: 4R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1690 -234993 -139.05 -658.24 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : -139.05 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.342

(partial model without unconserved sides chains): PDB file : Tito_4R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R0C-query.scw PDB file : Tito_Scwrl_4R0C.pdb: