Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQKRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKE-RYLQTHSTVLL-PESI 2MHE Chain:A ((1-74)) MPDKQLL-------HIVVGGELKDVAGV---------------EFRDLSKVEFVGAYPSYDEAHKAWKAKAQATVDNAHARYFIIHAHKLLDPSEG

General information: TITO was launched using: RESULT: Template: 2MHE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 219 -10148 -46.34 -140.94 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -46.34 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_2MHE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MHE-query.scw PDB file : Tito_Scwrl_2MHE.pdb: