Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVFCFRLDKKEESAMAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTALNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPEFLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTLEQLKEANADGYVDVVED--KYSLIHRQAEKE-LFPYLEKNKISFVPYFPLASGLLTGKYELGEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRVSVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF 5DAN Chain:A ((16-271)) -----------------------------LGLGTWGIGGFETPDYSRDEEMVELLKTAIKMGYTHIDTAEYYGGGHTEELIGKAIKD--FRREDLFIVSKVW--PTHLRRDD-----LLRSLENTLKRLDTDYVDLYLIHWPNPEIPLEETLSAMAEGVRQGLIRYIGVSNFDRRLLEEAISKSQEPIVCDQVKYNIEDRDPERDGLLEFCQKNGVTLVAYSPLRRTLLSEK--------------TKRT-----------------LEEIAKNHGATIYQIMLAWLLAKPNV-VAIPKAGRVEHLRENLKATEIKLSEEEMKLLD------

General information: TITO was launched using: RESULT: Template: 5DAN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1323 -61784 -46.70 -244.20 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -46.70 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_5DAN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DAN-query.scw PDB file : Tito_Scwrl_5DAN.pdb: