Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEFEKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRKRIGMVFQRPNPFSK-SIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDKSALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNL-KEDYTIIIVTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG 2OLJ Chain:A ((34-263)) ------------------------------FGSLEVLKGINVHIREGEVVVVIGPSGSGKSTFLRCLNLLED-----FDEGEIIIDGINLKAKDTNLNKVREEVGMVFQRFNLFPHMTVLNNITLAPMKVRKWPREKAEAKAMELLDKVGLKDKAHAYPD----SLSGGQAQRVAIARALAMEPKIMLFDEPTSALDPEMVGEVLSVMKQLANEGMTMVVVTHEMGFAREVGDRVLFMDGGYIIEEGKPEDLFDRPQHERTKAFLSKVF-

General information: TITO was launched using: RESULT: Template: 2OLJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1147 -36883 -32.16 -161.77 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -32.16 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.537

(partial model without unconserved sides chains): PDB file : Tito_2OLJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLJ-query.scw PDB file : Tito_Scwrl_2OLJ.pdb: