Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGKMIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVS-SSFFSQKFQKNIEEILVAPV-PTHVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHV--YSWFMVIITLLMTAILFSLAGLLNGIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGFLGTID-------------------------VPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS 5DO7 Chain:D ((395-671)) --PGAVQQFTTLIRRQISNDFRDLP-TLLIHGAEACLMSMTIGFLYFGHGSI----QLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKP-

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 997 -162417 -162.91 -654.91 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : -162.91 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.253

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: