Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDIMKRE-----SVSLKPPYPI-LGVEVAGEVVENKSDISTFTPGTKVYGLVNL----GGYAEYAVMPADRAILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQLSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGASYWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLI----GKRVQLLGTLLTPRSDQYKAELAQTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE 3TQH Chain:A ((8-320)) -KAIQFDQFGPPKVLKLVDTPTPEYRKNQMLIKVHAASLNPIDYKTRNGSGFVAKKLKNNLPSGLGYDFSGEVIELGSDVNNVNIGDKVMGIAGFPDHPCCYAEYVCASPDTIIQKLEKLSFLQAASLPTAGLTALQALNQ-AEVKQGDVVLIHAGAGGVGHLAIQLAKQ--KGTTVITTASKRNHAFLKALGAEQCINYHEEDFLLAI-----STPVDAVIDLVGGDVGIQSIDCLKETGCIV--------SVPTITAGRVIEVAKQKHRRAFGLL-----KQFNIEE----LHYLGKLVSEDKLRIEISRIFQLSEAVTAHELLETGHVRGKLVFKV--

General information: TITO was launched using: RESULT: Template: 3TQH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1773 -2693 -1.52 -9.01 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -1.52 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.572

(partial model without unconserved sides chains): PDB file : Tito_3TQH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TQH-query.scw PDB file : Tito_Scwrl_3TQH.pdb: