Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRIVYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEYTIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESSTENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK 3FN2 Chain:B ((45-69)) --------------------------------------------------------------------------------------------------------------------RYYGRGYVRITDKDGQVITYEDGSV---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FN2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 23 -2102 -91.39 -84.08 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.52 3D Compatibility (PKB) : -91.39 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.191

(partial model without unconserved sides chains): PDB file : Tito_3FN2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FN2-query.scw PDB file : Tito_Scwrl_3FN2.pdb: