Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTDTALIMALPNESKGLFE-QA--------------------GIEVHYSGIGKINAAFKAFEVIQKTGCKTLINLGTAGSSS--FNRHDLVEIKTFVQRDMDVSPLGFEVGV-----TPLDDHLAAEIHLQT----HFADLPKGICGTGDSFETGQPKV--------ACDVVDMEGYALAKVCHKLGVRLISVKYITDGADDTAHLDWEENLLLGAQKLLALYQNHF 1ZOS Chain:E ((1-225)) --MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVVLVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDVTAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEAIKSHFPEVLAVEMEGAAIAQAAHTLNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRSAQVLLAF-

General information: TITO was launched using: RESULT: Template: 1ZOS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 915 -21212 -23.18 -114.66 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain E : 0.71 3D Compatibility (PKB) : -23.18 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.435

(partial model without unconserved sides chains): PDB file : Tito_1ZOS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZOS-query.scw PDB file : Tito_Scwrl_1ZOS.pdb: