Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIAIIGAGISGLMSALELVEQGCTISIFDQQQ-A-GQAASWAGGGILSPMYPWRYVHAVNQLAQFGKASYQAWNQKLYPVTGIDFEIHDTGMLIFDEEDFDVGL-SYAEQHQEPIQRCEYLQRDALEQVNPHISE-QFQEAIYFPELSNIRNPRVLQSLISYLKQHPNVEFFEH-AAVKKLIQQGDVIQALQTEDGRKHTADHFVITSGAWSHYWNSQLKLEIPVEPVQGQMLLFKTPAHW-----LPT-M-CM--NRVMYLIPRMD-GHIVCGSSMAHR---G--FDT--STDETTQHNILEACLEMVPELADFPIVHRWAGLRPSSPNGVPYIGKMPEMDNLWANFGHFRNGLCMGAGSAQLLRQLMLGQPTLVDAKAYSPERLQNKILA 2GF3 Chain:B ((4-382)) FDVIVVGAGSMGMAAGYQLAKQGVKTLLVDAFDPPHTNGSHHGDTRIIRHAYGEG--REYVPLALRSQELWYELEKET---H--HKIFTKTGVLVFGPKGESAFVAETMEAAKEHSLTVDLLEGDEINKRWPGITVPENYNAIFEPNSGVLFSENCIRAYRELAEARG-AKVLTHTRVEDFDISPD-SV-KIETAN-GSYTADKLIVSMGAWNSKLLSKLNLDIPLQPYRQVVGFFESDESKYSNDIDFPGFMVEVPNGIYYGFPSFGGCGLKLGYHTFGQKIDPDTINREFGVYPEDESNLRAFLEEYMPGAN-GELKRGAVCMYTKTLDEHFIIDLHPEHSNVVIAAGFSGHGFKFSSGVGEVLSQLALTGKTEHDISIFSINRPALKE--

General information: TITO was launched using: RESULT: Template: 2GF3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2250 -36498 -16.22 -102.23 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -16.22 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_2GF3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GF3-query.scw PDB file : Tito_Scwrl_2GF3.pdb: