Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEEGLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALLMEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREKDGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQM-VDKVIVAGRDGVQISTSKKGK 1O8B Chain:A ((23-218)) --------------------IVGVGTGST------------------EGAVSSSDA------------FDLNEVDSLGIYVDGADEINGHMQMIKGGG-ALTREKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQ----GVVTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV-----

General information: TITO was launched using: RESULT: Template: 1O8B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 934 -77950 -83.46 -466.77 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -83.46 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.398

(partial model without unconserved sides chains): PDB file : Tito_1O8B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O8B-query.scw PDB file : Tito_Scwrl_1O8B.pdb: