Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQSGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAV-VFPVLHGPMGEDGSVQGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEVEEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGV-NAREIEVGLLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFRAIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDLAKESFDKREAHLI 4EG0 Chain:A ((16-313)) ----VAVLFGGESAEREVSLTSGRLVLQG------------LRDAG--IDAHPFDPA----ERPL-------------SALKDEGFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGVPTPPFETVMRGDDYAARATDIVAKLGLPLFVKPAS-----AVLKVKTADALPAALSEAATHDKIVIVEKSIEGGGEYTACIAGDLDLPLI---KIVP---------------TQYLIPCGLPAEQETELKRIARRAFDVLGCTDWGRADFMLDAAGNAYFLEVNTAPGMTDHSLPPKAARSIGIGYSELVVKVLSL-------------

General information: TITO was launched using: RESULT: Template: 4EG0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1522 -14741 -9.69 -53.41 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -9.69 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_4EG0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EG0-query.scw PDB file : Tito_Scwrl_4EG0.pdb: