Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAEL-DKEKPVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL 3G91 Chain:A ((4-256)) LKIISWNVNGLRAVH---------RKGFLKWFMEEKPDILCLQEIKAAPEQLPRKLRH-----VEGYRSFFTPA---ERKGYSGVAMYTKVPP-SSLREG-FGV-ERFDTEGRIQIADFDDFLLYNIYFPNGAMSEERLKYKLEFYDAFLEDVNRERDSGRNVIICGDFNTAHREIDLARPKENSNVSGFLPVERAWIDKFIENGYVDTFRMFNSD-PGQYTWWSYRTRA-RERNVGWRLDYFFVNEEFKGKVKRSWILSDVMGSDHCPIGLEIE-

General information: TITO was launched using: RESULT: Template: 3G91.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1367 -68575 -50.16 -272.12 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -50.16 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_3G91.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G91-query.scw PDB file : Tito_Scwrl_3G91.pdb: