Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQVDIAALSPQVVWQHFQTLCTIPRPSKHEQQLREFLQNWAESRNLETYVD----------------------EVGNLIIRKNATPGKKHVSGVILQGHLDMVTQANTGTVHDFFKDPIRPVLEEGWLIAKDTTLGADNGIGVALALAVLDSND-----IHGPIEVLLTVDEEAGMSGAR-LLETGVLKGKWLFNIDTEEWGELYLGCAGSIDVEVEQPLNYEPIPENLNIVNIQVAGLKGGHSGVDIHLGRGNANVILARFLNQHLASLGGHLVEFTGGTARNALPREAVATIAISPNQLSSLEKLLAEYQAAWKEQLKGIDDNLQLSIQSTGAKVTEVINQQQQNEWLQALATSPYGVASMSQVLPDVVETSNNIGVVRLNREGGKAILMVRSMVNQEAQDFAEKIQAHFSQFN----------IGSTLT-PLVSGWTPNPDSAALKCLQQAYQNAFNIEPNLKVIHAGLECGIIAEHYPHLQMVSFGPDIQGAHAPGERVKVDTVEKCWKLLVTALASVE 3PFO Chain:B ((22-427)) -------DRNFNDQVAFLQRMVQFRSVRGEEAPQQEWLAQQFADRGYKVDTFSLADVDIASHPKAAPMDTIDPAGSMQVVATADSD---GKGRSLILQGHIDVVPEGPV---DLWSDPPYEAKVRDGWMIGRGA---QDMKGGVSAMIFALDAIRTAGYAPDARVHVQTVTEEESTGNGALSTLMR-GYRADACLIPEPTG-HTLTRAQ------------------VGAVWFRLRVRGT-PVHVAYS-E-TGTSAILSAMHLIRAFEEYT-KEL---NA----QA-----------V--RDPWFGQVKN-------------PIKFNV--GI-----------------------IKGG---------------DWASSTA---AWCELDCRLGLLTGDTPQEAMRGIEKCLADAQATDSFLSENPAELVWSGFQADPAVCEPGGVAEDVLTAAHKAAFNAPLDARLSTAVNDTRYYSVDY-GIPALCYGPYGQGPHAFDERIDLESLRKTTLSIALFVAEW-

General information: TITO was launched using: RESULT: Template: 3PFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1927 -9688 -5.03 -26.40 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -5.03 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_3PFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFO-query.scw PDB file : Tito_Scwrl_3PFO.pdb: