Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRTANEFLTPQAIKVEAVSGTSAKVILEPLERGFGHTLGNALRRILLSSLPGAAVVEVEIEGVEHEYSTLEGLQQDIVELLLNLKGLSIKLFDQNEAYLTLEKQGPGDITAADLRLPHNVEVVNPEHLIGTLS-ATGSLKMRLKVSQGRGYETSD-SRFPEGETRPVGRLQLDASYSPIKRVSYTVENARVEQRTDLDKLVIDLETNGTVDPEEAIRKAATILQQQIAIFVDLQKDQTPVAQEPREEVDPILLRPVDDLELTVRSANCLKAENIYYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASKGLQLGMRLENWPPASLRMDDRFAYRSR 1BDF Chain:B ((6-233)) -----TEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRAILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH---DERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVD--------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1BDF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1083 -39346 -36.33 -176.44 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -36.33 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_1BDF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1BDF-query.scw PDB file : Tito_Scwrl_1BDF.pdb: