Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAVNASFTDYKVADISLADYGRKEIKLAEAEMPALIGLRKRHAASKPLAGAKILGCIHMTIQTAVLIETLVELGAEVRWTSCNIFSTQDHAAAAIATRGIPVFAWKGETEEEYVWCLEQQI---NVNGQPWDANMILDDGGDLTALVH---------------------EKYPALLER----------------IHGITEETTTGVQRLIEMWKDGTLKVPAINVNDSVTKSKNDNKYGCRHSLNDAIKRATDMLLSGRRALVIGYGDVGKGSAQSLRQEGMVVRVTEVDPICAMQACMDGYEVVSPYKNGVQTGKKEDINHDLLGNTDLVVTTTGNYHVCDAAMLDSLKAGAVVCNIGHFDTEIDTAYLRGYKWVEVKPQVHQVYRSEDENNYLILLSEGRLVNLGNATGHPSRVMDGSFANQVLGQIHLFQEKFADLPASEKAAKIRVEVLPKKLDEEVAAAMVAGFGGVLTQLTQEQADYLGVAVEGPFKSDAYKY 5M65 Chain:B ((12-479)) ------GFTDYIVKDIALADFGRKEISLAETEMPGLMATREEYGPKQPLKGARIAGSLHMTIQTAVLIETLAALGADIRWVSCNIYSTQDHAAAAIAAAGIPVFAVKGETLTEY-WDYTAKLFDWHGGGTP---NMILDDGGDATMLVHAGYRAEQGDTAFLDKPGSEEEEIFYALVKRLLKEKPKGWFAEIAKNIKGVSEETTTGVHRLYEMANKGTLLFPAINVNDSVTKSKFDNLYGCRESLVDGIRRGTDVMLSGKVAMVAGFGDVGKGSAASLRQAGCRVMVSEVDPICALQAAMEGYEVVT--------------MEDAAPRADIFVTATGNKDIITIEHMRAMKDRAIVCNIGHFDNEIQIASLRNLKWTNIKPQVDEI--EFPDKHRIIMLSEGRLVNLGNAMGHPSFVMSASFTNQTLAQIELFANN-----KDSKYAK-KVYVLPKTLDEKVARLHLAKIGVKLTELRKDQADYIGVKQEGPYKSDHYRY

General information: TITO was launched using: RESULT: Template: 5M65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2614 5215 1.99 12.18 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : 1.99 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.460

(partial model without unconserved sides chains): PDB file : Tito_5M65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M65-query.scw PDB file : Tito_Scwrl_5M65.pdb: