Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISSAKKREVATRPDALPRTSVTEIFVELCLLFKIGEGGNKMAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPTERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL 3R2D Chain:J ((5-82)) --------------------------------------------EKIRIKLRAYDHRLLDQSVKQIIETVKRTGGVVKGPIPLPTRKSEFS---------------------RILDIIRFTPQTIEALMEISLPAGVDVEVKM

General information: TITO was launched using: RESULT: Template: 3R2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 310 -39919 -128.77 -511.78 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain J : 0.77 3D Compatibility (PKB) : -128.77 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.674

(partial model without unconserved sides chains): PDB file : Tito_3R2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R2D-query.scw PDB file : Tito_Scwrl_3R2D.pdb: