Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAESTTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEYAPKAVENF--MTHAKDGY-YNGLTFHRVIKDFMIQGGD-PKGDGTGGESIWGEGFETEINNHLYNIRGALSMARSQDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGMDVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK 1VDN Chain:A ((16-148)) ------------------------------------------------------------------------------GRVVFKLYNDIVPKTAENFRALCTGEKGFGYAGSPFHRVIPDFMLQGGDFTAGNGTGGKSIYGGKFPDENFKKHHDRPGLLSMANA-GPNTNGSQFFITTVP------------------------CPWLDGKHVVFGEVVDGYDIVKKVESLGSPSG---------------------

General information: TITO was launched using: RESULT: Template: 1VDN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 623 14297 22.95 110.83 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 22.95 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_1VDN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VDN-query.scw PDB file : Tito_Scwrl_1VDN.pdb: