Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRMHLPTLLKNKEKSQRSRQMSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRVA-EIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIK-DGQLPQGTDLLRISQNRIFEKDFLSNKSQVTVAPYKVVTSSLDLADI--DLSKNYVLKTATGGYDGHGQKVIRSEADLEAAYALAD--SADCVLEEFVNFDLEISVIVSGN-GKEVTFFPVQENIHRNNILSKTIVPARISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEACDFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIEAKHNRKMGHVTLFSDVPDSVDELGEGIDF 4MAM Chain:A ((1-356)) ------------------------MKIGIIGAGQLARMLSLAGTPLGLEFHCLGKNGDCAE-EVVKTVTDIELTKVNDVVAWAKQFDVITFENENISHELIKAINHEVSV-YPSAKAIAISQDRLLEKSFMQ-DHGIATAKFVNIDSLAKLQSAVDDHGLPAILKTRRFGYDGKGQFVIRSQEDITKAWDVLKDAPDGLIYEAFVDFDYEVSQICTADLKGNIAFYPLARNTHKQGIIVESEAPF-ENVVLAEKAQQIAKILVKEFAYVGTLAIEFFVKGDELIVNEIAPRVHNSGHWSIDGAVTSQFENHVRAIAGLILGDTTS-RKTVMLNCIGGMPA--TKDLAALDRVKIHSYNKE-PRKGRKVGHLNLNLNDETDE-YQLLQVK-

General information: TITO was launched using: RESULT: Template: 4MAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2019 10660 5.28 30.54 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 5.28 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_4MAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MAM-query.scw PDB file : Tito_Scwrl_4MAM.pdb: