Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEEGLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALLMEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREKDGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDK-VIVAGRDGVQISTSKKGK 5UF2 Chain:A ((13-230)) -DELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSGK---KIKGAVSTSKKSGELLARYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHLNEKIVASASEKFVCIADESKYVSRLGKFPLPVEAVESARSLVSRKLLAMGGQPELRI----GYTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVIYPCQ--

General information: TITO was launched using: RESULT: Template: 5UF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1289 -97236 -75.43 -448.09 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -75.43 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_5UF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UF2-query.scw PDB file : Tito_Scwrl_5UF2.pdb: