Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRPTVLCFSGLDPSGGAGLQADIEAIGQSGAHAAIACTALTIQNSQQVFGSEATSKELLLAQANAVVGDLPIKCVKSGMLGTTDNIAALAEFLRAH--PDYQYVLDPVLVANSGGSLGDQATLVKAFVELIL-LATLITPNTVELRALTGVT-------DLDQATQKLFE-MGAKAVLVKGGHEDTP-DFIKNSLYIDGE-LAASSTCPRLE-GEYHGSGCSLASFIAGRLALGDSLKIAVQHAETWLFGVLKNAETPVPNGQKIPKRF 3RM5 Chain:A ((22-291)) -LPTVLSIAGTDPSGGAGIEADVKTITAHRCYAMTCITALNAQTPVKVYSINNTPKEVVFQTLESNLKDMKCNVIKTGMLTAA-AIEVLHEKLLQLGENRPKLVVDPVL---------G-KDIVSLITEKVAPFADILTPNIPECYKLLGEERKVNGLQDIFQIAKDLAKITKCSNILVKGGH----EKYITDVLFLGAEQKFIIFKGNFVNTTHTHGTGCTLASAIASNLARGYSLPQSVYGGIEYVQNAVAIGCD------------

General information: TITO was launched using: RESULT: Template: 3RM5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1233 -69176 -56.10 -304.74 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -56.10 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_3RM5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RM5-query.scw PDB file : Tito_Scwrl_3RM5.pdb: