Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKPYLIAPSILSADFARLGEDVDKVLAAGADVVHFDVMDNHYVPNLTFGAGVCKALKNYGITAPVDVHLMVSPVDRIIGDFLEAGADIITFHPEASH--HIDRSLQLIKSGGAKAGLVFNPATPLHYLDHVLDKVDQILLMSVNPGFGGQKFIPMTLEKLREARKIIDASGRDIRLEVDGGVGPANIREIAEAGADMFVAGSAIFGQPDYKVMIDQMRAELAKVGSVDA 1TQJ Chain:D ((3-223)) --KNIVVAPSILSADFSRLGEEIKAVDEAGADWIHVDVMDGRFVPNITIGPLIVDAIRPL-TKKTLDVHLMIVEPEKYVEDFAKAGADIISVHVEHNASPHLHRTLCQIRELGKKAGAVLNPSTPLDFLEYVLPVCDLILIMSVN-----QSFIPEVLPKIRALRQMCDERGLDPWIEVDGGLKPNNTWQVLEAGANAIVAGSAVFNAPNYAEAIAGVRNSKRP------

General information: TITO was launched using: RESULT: Template: 1TQJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1206 -69576 -57.69 -325.12 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -57.69 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.677

(partial model without unconserved sides chains): PDB file : Tito_1TQJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TQJ-query.scw PDB file : Tito_Scwrl_1TQJ.pdb: