Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAVNASFTDYKVADISLADYGRKEIKLAEAEMPALIGLRKRHAASKPLAGAKILGCIHMTIQTAVLIETLVELGAEVRWTSCNIFSTQDHAAAAIATRGIPVFAWKGETEEEYVWCLEQQI---NVNGQPWDANMILDDGGDLTALVH---------------------EKYPALLER----------------IHGITEETTTGVQRLIEMWKDGTLKVPAINVNDSVTKSKNDNKYGCRHSLNDAIKRATDMLLSGRRALVIGYGDVGKGSAQSLRQEGMVVRVTEVDPICAMQACMDGYEVVSPYKNGVQTGKKEDINHDLLGNTDLVVTTTGNYHVCDAAMLDSLKAGAVVCNIGHFDTEIDTAYLRGYKWVEVKPQVHQVYRSEDENNYLILLSEGRLVNLGNATGHPSRVMDGSFANQVLGQIHLFQEKFADLPASEKAAKIRVEVLPKKLDEEVAAAMVAGFGGVLTQLTQEQADYLGVAVEGPFKSDAYKY 5M65 Chain:A ((11-479)) -----PGFTDYIVKDIALADFGRKEISLAETEMPGLMATREEYGPKQPLKGARIAGSLHMTIQTAVLIETLAALGADIRWVSCNIYSTQDHAAAAIAAAGIPVFAVKGETLTEY-WDYTAKLFDWHGGGTP---NMILDDGGDATMLVHAGYRAEQGDTAFLDKPGSEEEEIFYALVKRLLKEKPKGWFAEIAKNIKGVSEETTTGVHRLYEMANKGTLLFPAINVNDSVTKSKFDNLYGCRESLVDGIRRGTDVMLSGKVAMVAGFGDVGKGSAASLRQAGCRVMVSEVDPICALQAAMEGYEVVT--------------MEDAAPRADIFVTATGNKDIITIEHMRAMKDRAIVCNIGHFDNEIQIASLRNLKWTNIKPQVDEI--EFPDKHRIIMLSEGRLVNLGNAMGHPSFVMSASFTNQTLAQIELFANN-----KDSKYAK-KVYVLPKTLDEKVARLHLAKIGVKLTELRKDQADYIGVKQEGPYKSDHYRY

General information: TITO was launched using: RESULT: Template: 5M65.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2602 -1567 -0.60 -3.65 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : -0.60 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_5M65.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M65-query.scw PDB file : Tito_Scwrl_5M65.pdb: