Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALIVQKYGGTSMGTPERILNVARRVKRWHDHGHKVVVVVSAMSGETNRLLALAKAIT---------------------------------------------------------ETPDPREIDQMVSTGEQVTISMLAMALNSIGVEAKSYTGRQVGIKTDSAFTKARIESID-TDVMTNDLDAGRVIVVAGFQGFDANGNTTTLGRGGSDTSGVAIAAALKADECQIYTDVDGVYTTDPRVAPKAKKIDRISFEEMLEMASLGSKVLQIRSVEFAGKYQVPLRVLSSFDNDDDGAFDDEFKENVGTLITTEAEDTMEQPIIAGIAFNRDEAKLTILGVPDE--PGIASKILSPVSDANIEVDMIVQNVEEDGTTDFTFTVNRVDLAKAEKILNETAKN-I---GAREVSTRDDIVKVSIVGVGMRSHAGVASKMFTALADEGINILMIS--TSEIKVSVIIDEKYLELAVRCLHTAFGLDREHGESSARA 2HMF Chain:B ((1-468)) --TTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVRDIAKVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPRLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPE-----------SEGTLITNDME--MSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSET---NISLVVSEEDVDKALKALKREFGDFLNNNLIRDVSVDKDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKFIE-----------

General information: TITO was launched using: RESULT: Template: 2HMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2170 2194 1.01 5.53 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 1.01 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_2HMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HMF-query.scw PDB file : Tito_Scwrl_2HMF.pdb: