Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAVAY--QKAGPITLPEALVDIELDTPVAKGHDLLIRVQAVSVNPVDTKIRKNVSAE----------QSGWKVLGWDAVGTVEAIGDKVTQFKIGDVVWYAGAL--------------------------NRQGSNSELQLVDERIVGHKPKTLEATEAAALPLTAITAWEMLFDR--LQVPKTAPANTTILVIGGAGGVGSITIQLLKQLTNLTIIATASRPETKEWVEQLGADYVLDHRQ-SLAAQ-----------------------IKL-LG-LSAPLYVFSTTETDQHLSDIVELIAPQGHFGLIDDPDQLD--I--KPFKSKSVSVHWEFMFTRSMFQTEDMEKQSALLNEVSKLVDEGKIKTTVTQVLSPINAENLTRVHEQIESGST-KGKIVLHGF 4Y1B Chain:A ((41-422)) MYASLIRQERYGRP--VDAFRTEVVDVPPVGPGQVLVKVMAAGVNYNNVWAALGEPLDVIAARQKQGATEDFHIGGSDLSGIVWAVGDGV-RLKPGAEVVVLACRWDESAQDIRLGADPVTSSTQRVWGYEENYGSFAQFAVVDEYMCHPKPQRLSWAAASCYMATAATAYRQLFGWEPHTVR---P-GDPVLIWGGAGGLGSIAIQLVRHVG-GIPVAVVSSEERGEFCMRLGAKGWIDRREFDHWGRLPDTTDEEAMKQWLDGARAFGRRFWEVLGERRAPRIVLEHSGA-DTIPTSIYMADNAGMVVICGGTTGYNGDVDLRFLWMRQKRLQG------------SHAASAREAREITRLIDQGAIDPCLSRTF-GF--EEIGLAHQLIHDNQHPSGNMAVLV-

General information: TITO was launched using: RESULT: Template: 4Y1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1634 -11161 -6.83 -35.89 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -6.83 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.542

(partial model without unconserved sides chains): PDB file : Tito_4Y1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y1B-query.scw PDB file : Tito_Scwrl_4Y1B.pdb: