Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEKPVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL 5EWT Chain:A ((1-247)) MKIVSWNVNGIRAALK---------KNLIDFIENNMFEVIMFQETKGDIVPL-----DF---IMMGYEVISFPA---KRKGYSGVMTLTKIKP-INVIKGL-QI-KEFDDEGRTVTLELKDFYVINAAFPRAGDNLERLDFKLKFNNEIENFVLKLRRAKPVILCGDFNIAHQNIDGAFSDP--TIPGLTPQERSWFSHFLSLGFIDTFRYLHPN-VRKYSWWSYMGKA-REKNLGLRLDYCIVSEELKDRIKMADILIDIQGSDHAPIILELT-

General information: TITO was launched using: RESULT: Template: 5EWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1394 -122360 -87.78 -495.38 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -87.78 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.445

(partial model without unconserved sides chains): PDB file : Tito_5EWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EWT-query.scw PDB file : Tito_Scwrl_5EWT.pdb: