Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLRIEKNNGIATVYLNRPDKRNAMSFALLKELVTTAKAIQKDRDIRCVILTGEAQVFSAGIDLSDLNNPKNSAFAIWELVKPGQSLFQKAFLIWQNLPVPVIAALEGYCFGAGMQLALAADIRIAHPDTKMSIMESRWGLVPDMGLTRSLKGLIGVDLAKELTLTARVLDGNYAKDIGLVTHLSE--NPLEKANAIASEMLQRSPDALTAAKRVLDAMEHQPE-KSLRLEKIWQLKLLLGKNSKLARKKDK---HPEVKFLPRQYR 3HIN Chain:A ((17-266)) --TLVVDTVGPVLTIGLNRPKKRNALNDGLMAALKDCLTDIP--DQIRAVVIHGIGDHFSAGLDLSELRERDATEGLVH-----SQTWHRV-FDKIQYCRVPVIAALKGAVIGGGLELACAAHIRVAEASAYYALPEGSRGIFVGGGGSVRLPRLIGVARMADMMLTGRVYSAAEGVVHGFSQYLIENGSAYDKALELGNRVAQNAPLTNFAVLQALPMIAEANPQTGLLMESLMATVAQSDQEAKTRIRAFLDHKTAKV--------

General information: TITO was launched using: RESULT: Template: 3HIN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1230 -119094 -96.82 -488.09 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -96.82 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_3HIN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HIN-query.scw PDB file : Tito_Scwrl_3HIN.pdb: