Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQSNMQNKFLIDAEIGDDDVTLPNLPAIDVPIVESPVKQEVKAEEVVEAAPSTEPEQPKSGFFGRMKEGLSKTRRNFTDGMVNILIGGKEIDDELLEEVEGQL--------LVADIGVDATKTIITNLTERTARGDLIYSHSLYKALQEELVALLAP--RVKPLH--IDPNKS--PYVILMVGVNGVGKTTTIGKLAKRLQGEGKKVMLAAGDTFRAAATEQLQI-------------WGERNDIAVVAQGHGADSASVIFDASESARAKGIDVLIADTAGRLHNKSNLMEELKKVKRVMQKIDATAPHEIMLVVDAGTGQNAINQVQEFDQAVG----------LTGITITKLDGT-AKGGVLFNIASRTHVPIRFIGVGEKIDDLRPFSAKSFVAALFETEK 5L3Q Chain:D ((332-637)) -------------------------------------------------------------------------------------------SLSREDMESVLDKMRDHLIAKNVAADIAVQLCESVANKLEGKVMGTFSTVTSTVKQALQESLVQILQPQRRVDMLRDIMDAQRRQRPYVVTFCGVNGVGKSTNLAKISFWLLENGFSVLIAACDTFRAGAVEQLRTHTRRLSALHPPEKHGGRTMVQLFEKGYGKDAAGIAMEAIAFARNQGFDVVLVDTAGRMQDNAPLMTALAKL------ITVNTPDLVLFVGEALVGNEAVDQLVKFNRALADHSMAQTPRLIDGIVLTKFDTIDDKVGAAISMTYITSKPIVFVGTGQTYCDLRSLNAKAVVAALMK---

General information: TITO was launched using: RESULT: Template: 5L3Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1243 -35521 -28.58 -132.54 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -28.58 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.610

(partial model without unconserved sides chains): PDB file : Tito_5L3Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L3Q-query.scw PDB file : Tito_Scwrl_5L3Q.pdb: