Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNLTLSQFL--QQEKGNLTPELAQVIDTIAATCKTIDQALQKGALAGILGSAGNENVQGETQKKLDVISNDYLIDALKVHPHVGGLASEELDD--FTPAQENGEYLVLFDPLDGSSNIDINMCVGTIFSILPAKNAVTQA--QDFMQAGTQQVAAGYVLYGPSTMMALTVGNGVAFFTLDPETQTFLLTTENVQVSADTQEFAINASNQRHWEQPVKQYIEELLAGKTSVREKDFNMRWVACMVGDVHRILCRGGIFLYPYDLKDPKKAGRLRLMYEANPMSMLIEQAGGASTTGRVRILEIEPTELHQRVPVIIGSKNEVERVTSYH 1FBH Chain:A ((11-324)) ---VTLTRFVMEQGRKARGTGEMTQLLNSLCTAVKAISTAVRKAGIA-------------DQVKKLDVLSNDLVINVLKSSFATCVLVTEEDKNAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSIGTIFGIY-RKNSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMVNGVNCFMLDPAIGEFILVDRNVKIKKKGSIYSINEGYAKEFDPAITEYIQRKKFPPDN--SAPYGARYVGSMVADVHRTLVYGGIFMYPANKKSPK--GKLRLLYECNPMAYVMEKAGGLATTGKEAVLDIVPTDIHQRAPIILGSPEDV-------

General information: TITO was launched using: RESULT: Template: 1FBH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1677 42834 25.54 145.20 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 25.54 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_1FBH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FBH-query.scw PDB file : Tito_Scwrl_1FBH.pdb: