Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALKFIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKYNTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSIVEQEDLDMPVGLY--DINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYNLIVCAMDTEDYPCSVNFPFTFKEGELADYYKD--WELVKYNENPGHLHRRDENGNRIQLRFATLLAKKIK 3LCC Chain:A ((13-228)) ----------------------------------------------------------------GGNVIPTPEEVATFLHKTVEEGGWEKCWEEEITPWDQGR--ATPLIVHLVDTS-SLPLGRALVPGCGGGHDVVAMASPERFVVGLDISESALAKANETYGSSPKAEYFSFVKEDVFTWRPTELFDLIFDYVFFCAIEPEMRPAWAKSMYELLKPDGELITLMYPITDHVG---GPPYKVDVSTFEEVLVPIGFKAVSVEENPHAIPTRKGKEKLGRWKKINL------

General information: TITO was launched using: RESULT: Template: 3LCC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1148 -34859 -30.36 -164.43 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -30.36 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.383

(partial model without unconserved sides chains): PDB file : Tito_3LCC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3LCC-query.scw PDB file : Tito_Scwrl_3LCC.pdb: