Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIIPAIDLKDGKCVRLKQGRMEDDTVFSDDLVATAQHWVNEGARRLHLVDLNGAFAGTPIHKPVVEAIAKAQPELPIQIGGGIRSLETIEHYLEAGVTFVIIGTKAVQEPEFVEEACKSF-AGHIIVGIDAMN----GMVATDGWANVTDVKATDLAKRFADAGVSSIVYTDIARDGMMQGVNVEQTVNLAQYSGLPVIASGGVTNLDDVRNLKGQLGILGAITGRAIYEGTLNLREAQLLLDENRL 4EVZ Chain:B ((4-246)) KRIIPCLDVKDGRVVKGVNFE--NLR-DAGDPVELAARYDEEGADELVFLDIT----GRETMLEVVERTAE-QVFIPLTVGGGIRSVEDASRLLRAGADKVSINTAAVKNPELITEAAEEFGSQAVVVAIDAKRVGGGWEVFTHGGRKPTGLDAVEWARKVVELGAGEILLTSMDRDGTKAGYDLELTRAVSEAVSVPVIASGGAGELEHFAEVFELEGADAALAASIFHFGEITIREVKAYLRERG-

General information: TITO was launched using: RESULT: Template: 4EVZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1402 -71310 -50.86 -304.74 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -50.86 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.663

(partial model without unconserved sides chains): PDB file : Tito_4EVZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EVZ-query.scw PDB file : Tito_Scwrl_4EVZ.pdb: