Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEEGLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALLMEKVVATPSKEYIWVVDESKLVEKLGA-FKLPVEVVQYGAEQVFRHFERAGYKPSFREKDGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQ-MVDKVIVAGRDGVQISTSKKGK 1M0S Chain:B ((4-218)) -LEMKKLAAQAALQYVKADRIVGVGSGSTVNCFIEALGTIKD---KIQGAVAASKESEELLRKQGIEVFNANDVSSLDIYVDGADEINPQKMMIKGGGAALTREKIVAALAKKFICIVDSSKQVDVLGSTFPLPVEVIPMARSQVGRKLAALGGSPEYRE----GVVTDNGNVILDVHNFSILNPVEIEKELNNVAGVVTNGIFALRGADVVIVGTPEGAKVI------

General information: TITO was launched using: RESULT: Template: 1M0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1287 -75342 -58.54 -353.72 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -58.54 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_1M0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M0S-query.scw PDB file : Tito_Scwrl_1M0S.pdb: